1.Honokiol has an obvious antifungal effect. The half-effective concentration （ECso） of honokiol to Rhizoctonia oryzae and Streptomyces graminis was 3.14~12.87 mg/kg; The minimum inhibitory concentration （MIC） for Trichophyton mentagrophytes, Microsporum gypsum, Epidermophyton flocculates, Aspergillus niger, Cryptococcus neoformans, and Candida albicans is 25-100 μ g /mL。 Honokiol has an obvious antibacterial activity against gram-positive bacteria and acid-resistant bacteria: the MIC （agar diffusion method） of honokiol against Bacillus subtilis, Staphylococcus aureus, and Mycobacterium smegmatis is 5 μ G/mL, and the MIC of magnolol is 7.5 μ g /ml； And honokiol also had a significant inhibitory effect on the pathogenic bacteria around the teeth, and the MIC of actinomycetes associated with actinomycetes, clostridium gingivalis, Bacteroides intermedius, Micrococcus luteus and Bacillus subtilis was 25 ug/mL; The structure-activity relationship study showed that biphenyl showed no activity, and the strong anti-caries effect of honokiol was due to the presence of hydrophilic allyl and lipophilic phenolic hydroxyl groups on the biphenyl ring, which made it adhere to the cells of Streptococcus mutans and other gram-positive bacteria, and honokiol also had strong antibacterial activity against Propionibacterium pustulosus and Propionibacterium granulosa. In addition, honokiol has a strong inhibitory effect on Escherichia coli and Streptococcus, and the antibacterial mass concentration is 10 μ Within g/mL.
2. Honokiol has an anti-inflammatory effect: honokiol can inhibit lymphocyte budding in a dose-dependent manner, and the half inhibitory concentration （IC50） is 7.7 μ GlmL, about dehydrocortitol （IC50 is 0.8 μ G/mL）; It can also significantly inhibit the rat ear swelling induced by picryl chloride, with the inhibition rate of 23.6％. In addition, honokiol can also reduce the permeability of the capillary wall around the inflammatory site, and inhibit the migration of white blood cells and the proliferation of fibrous tissue. Magnolia Officinalis extract, angelica extract, ginger extract, etc.）, standardized plant extract production workshop （eucommia extract, macleaya extract, rosemary extract, etc.）, plant effective component monomer separation and purification workshop （dihydromyricetin, amygdalin, myricetin, honokiol, and honokiol, etc.）
3.Honokiol has a calmodulin antagonistic effect: honokiol antagonizes the stimulation of calmodulin on cyclic nucleotide phosphodiesterase, the IC50 is 27 mol, indicating that honokiol causes the increase of free Ca2+in the cytoplasm through the phosphodiesterase c-mediated pathway, and this increase is due to the release of Ca2+from the intracellular storage.
